Transfection to stable cell line in a single step

piggyBac™ (PB) transposon reagents for stable cell line development & bioprocess

The piggyBac transposon is a powerful and simple non-viral technology for stable cell line engineering. For bioproduction applications piggyBac is especially enabling due to its speed and enhanced production levels of even difficult to express proteins. Transposagen piggyBac products include research use reagents and now Good Manufacturing Grade piggyBac mRNA for further manufacturing use. Applications and benefits of this technology have been demonstrated in over 550 peer-reviewed publications.

Graphic: Transposition Mechanism

3 Key Benefits:

  • Titer: ↑ titers (↓ future Cost of Goods)
  • Efficiency: Better use of existing resources
  • Speed: Faster to the clinic (bringing new therapies to patients faster)1 

Off-The-Shelf-Reagents

Super piggyBack transposase (sPBo) expression vector

Catalog #: SPB-DNA

Super piggyBac transposase. Sequence has been codon optimized

  • 10µg Price: $590.00
  • Availability: TBD
  • Research use only
  • Manufactured: N/A
  • Storage: ≤ -20C
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

Improved Super piggyBac tranpsposase mRNA

Catalog #: SPB-100

Sequence has been codon optimized (in comparison with SPB-003) to provide improved mRNA stability and protein expression in mammalian systems. The piggyBac transposase mRNA is recommended for cells that are not efficiently transfected with vector DNA.

  • 25µg Price: $690.00
  • Availability: June 2018
  • Research use only
  • Manufactured: N/A
  • Release assays: Concentration, Purity, Nucleic Acid Integrity, Potency
  • Storage: ≤ -60C
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

Good Manufacturing Grade super piggyBac transposase mRNA

Catalog #: SPB-200

Sequence has been codon optimized (in comparison with SPB-003) to provide improved mRNA stability and protein expression in mammalian systems. The piggyBac transposase mRNA is recommended for cells that are not efficiently transfected with vector DNA. It is ideally suited for applications where cell lines are to be used for downstream manufacturing i.e research and master cell banks, where GMP starting materials are required.

  • 35µg Price: $1,750.00
  • Availability: February 2018
  • For further manufacturing use
  • Manufactured: 1/11/2018
  • Release assays: Concentration, Purity, Nucleic Acid Integrity, Potency, Residual template DNA, Residual E. coli DNA, Sterility, Endotoxin, B&F, adventitious viruses
  • Storage: ≤ -60C
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

Excision-only piggyBac transposase expression vector, 10µg size (PBx-10)

Catalog #: SPB-002

Excision-only piggyBac transposase (PBx) expression vector is a transposase for excision of piggyBac selection cassettes and transgenes for Footprint-Free™ gene editing or phenotype reversions.

  • 10ug Price: $369.00
  • 25ug Price: $830.00
  • 50ug Price: $1,476.00
  • Availability: In stock
  • Research Use Only
  • Storage <-20C
  • This vector is non-renewable, and cannot be propagated in bacteria.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

piggyBac transposon base vector

Catalog #: SPB-007

This customizable piggyBac transposon vector contains an insulator-flanked large multiple cloning site (MCS), allowing the flexibility to clone in your promoter and DNA cargo of choice. The core insulators protect cargo from genomic position effects and gene silencing.

  • 10µg Price: $499.00
  • Availability: In stock
  • Research Use Only
  • Storage: <-20C
  • This vector is renewable (Ampicillin resistance)
  • Estimated delivery is 3-7 business days.

Production of sufficient quantities of antibody early in the development process to allow for testing in an expanding collection of biological and physicochemical assays is challenging. Transient gene expression in CHO cells allows for the rapid generation of small quantities of material. Although this can be rapid, scaling up to provide gram quantities of material can be prohibitive. Switching to stable gene expression is preferred for generating these large quantities of protein but the generation of clonal CHO cell lines can be both time consuming and labor intensive. More recently stable CHO cell pools have been utilized which can reduce the time to produce protein but may be generally less stable than clonal lines and may also have lower expression titers.

Transposagen has developed a piggyBac transposon system that has been validated for use in the generation of antibodies from CHO cell pools and individual clones. In these collaborative studies, published by Eli Lilly and others, antibody titers as high as 7.6g/L were obtained from a heterogenous CHO cell population. Titers were improved for each of the four antibodies tested in comparison with control pools. On average the fold increase for the three mono-functional antibodies was approximately 4-fold and for the bi-functional antibody tested was nearly 12-fold (specific productivity ranged from 4.2-9.3-fold improvement with the piggyBac pools)2. In addition, the PB system significantly increased the frequency of high titer clone isolation suggesting that the isolation of high producing stable CHO clones could be accelerated. Further studies have shown that the product quality attributes for protein obtained from the piggyBac system are identical to that from standard techniques; the system can be readily adapted to growth in bioreactors (demonstrated at 36L scale) and the PQA are stable over multiple passage (55 generations)3.

Therefore, these data demonstrate that the piggyBac transposon system is superior to standard methods for generation of CHO cell pools and would also make clonal isolation more efficient. Improved protein production and acceleration of clonal CHO cell generation make this system ideal for early development and may reduce the time for entry into the clinic significantly.

  1. Gavin Barnard, Eli Lilly & Co. Transposon-Mediated Gene Integration – The Future Of Stable CHO Cell Line Development? 25th ESACT Meeting; Lausanne; 14 May 2017

  2. Rajendra et al. (2016) Generation of Stable Chinese Hamster Ovary Pools Yielding Antibody Titers of up to 7.6 g/L Using the piggyBac Transposon System. Biotechnol. Prog., Vol. 32, No. 5

  3. Rajendra et al. (2017) Bioreactor scale up and protein product quality characterization of piggyBac transposon derived CHO pools. Biotechnol Prog. Mar;33(2):534-540