piggyBac® (PB) transposon system for stable transfection & Footprint-Free® gene editing

The piggyBac transposon is a powerful and simple non-viral technology with many applications in genome engineering. Due to its large DNA cargo capacity (>200kb) and stability piggyBac has been used to engineer difficult cell lines and targets such as T-cells and ion-channels. When used in combination with CRISPR or other site-specific nucleases, piggyBac enables Footprint-Free gene editing, as little as a single base-pair can be edited at high efficiency by selection, followed by seamless removal of the markers. Applications and benefits of this technology have been demonstrated in over 750 peer-reviewed publications.

Graphic: Transposition Mechanism

Key Benefits:

  • Small to enormous gene integration (200KB+)
  • Efficiency: Better use of existing resources
  • High and stable expression
  • Seamless removal for Footprint-Free gene editing

How Footprint-Free Gene Editing Works

Graphic: How it Works

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Off-The-Shelf-Reagents

Super piggyBac transposase (sPBo) expression vector

Catalog #: SPB-DNA

Super piggyBac transposase. Sequence has been codon optimized

  • 25µg only Price: $1,330
  • Availability: TBD
  • Research use only
  • Manufactured: N/A
  • Storage: ≤ -20C
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

Improved Super piggyBac tranpsposase mRNA

Catalog #: SPB-100

Sequence has been codon optimized (in comparison with SPB-003) to provide improved mRNA stability and protein expression in mammalian systems. The piggyBac transposase mRNA is recommended for cells that are not efficiently transfected with vector DNA.

  • 25µg Price: $999.00
  • Availability: June 2018
  • Research use only
  • Manufactured: N/A
  • Release assays: Concentration, Purity, Nucleic Acid Integrity, Potency
  • Storage: ≤ -60C
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

Good Manufacturing Grade super piggyBac transposase mRNA

Catalog #: SPB-200

Sequence has been codon optimized (in comparison with SPB-003) to provide improved mRNA stability and protein expression in mammalian systems. The piggyBac transposase mRNA is recommended for cells that are not efficiently transfected with vector DNA. It is ideally suited for applications where cell lines are to be used for downstream manufacturing i.e research and master cell banks, where GMP starting materials are required.

  • 35µg Price: $1,750.00
  • Availability: February 2018
  • For further manufacturing use
  • Manufactured: 1/11/2018
  • Release assays: Concentration, Purity, Nucleic Acid Integrity, Potency, Residual template DNA, Residual E. coli DNA, Sterility, Endotoxin, B&F, adventitious viruses
  • Storage: ≤ -60C
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

Excision-only piggyBac transposase expression vector

Catalog #: SPB-002

Excision-only piggyBac transposase (PBx) expression vector is a transposase for excision of piggyBac selection cassettes and transgenes for Footprint-Free gene editing or phenotype reversions.

  • 25ug Price: $1,330
  • Availability: In stock
  • Research Use Only
  • Storage <-20C
  • This vector is non-renewable, and cannot be propagated in bacteria.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

piggyBac transposon base vector

Catalog #: SPB-007

This customizable piggyBac transposon vector contains an insulator-flanked large multiple cloning site (MCS), allowing the flexibility to clone in your promoter and DNA cargo of choice. The core insulators protect cargo from genomic position effects and gene silencing.

  • 10µg Price: $999.00
  • Availability: In stock
  • Research Use Only
  • Storage: <-20C
  • This vector is renewable
  • Estimated delivery is 3-7 business days.