Transfection to stable cell line in a single step

Transposagen offers the industry’s most efficient non-viral stable transfection and transgenesis technology. PiggyBac™ has been used in many diverse applications and has been shown to work in nearly any species.

The piggyBac™ transposition mechanism

The piggyBac™ transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a “cut-and-paste” mechanism. During transposition, the piggyBac™ transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on each end of the transposon. Subsequent to ITR recognition, the piggyBac™ transposase excises ("cuts”) the transposon from the transposon vector which is then efficiently integrated (“pasted”) into random TTAA genetic locations.

Graphic: Transposition Mechanism

Application: Transgenesis

  piggyBac Retroviral Transient Transfection Stable Cell Generation FLiP-In
Cargo Size >250 kb ~4kb ~25kb ~25kb 250kb only one copy
Ease of cloning Easy easy easy easy easy
Parallel processing          
Time to result fast Two step; must make virus once cloning done Fast but fleeting Same as pBo Two step if making own flp cells
Stability of expression good Silencing common none variable Good if frt site is well placed
Safety concerns none Important none none none
Applicable to any cell? no yes no no no

What piggyBac™ is used for

piggyBac™ has been effective for mutagenesis in both cell lines and mice, transgenesis in a variety of species, modification of clinically relevant cell types, in vivo gene transfer, and production of biological therapeutics.

Graphic: What piggyBac™ is Used For

What piggyBac™ is Used For

The advantages of piggyBac

  • Large cargo capacity––over 200kb published
  • High, stable integration efficiency
  • Non-viral
  • Effective in a variety of genomes
  • Reversible
  • Can be used for Footprint-Free™ gene editing

piggyBac™ Reagents & Services

Choose any of our off-the-shelf expression vectors or contact us for a custom build.

Off-The-Shelf Reagents

sPBo-10
$369.00
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Super piggyBac™ transposase expression vector, CMV promoter, 10µg size (sPBo-10)

Catalog #: SPB-001-10

Super piggyBac™ transposase (sPBo) expression vector is a hyperactive piggyBac™ transposase that facilitates stable integration of small and large genes into target genomes.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 10µg size.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

sPBo-25
$830.00
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Super piggyBac™ transposase expression vector, CMV promoter, 25µg size (sPBo-25)

Catalog #: SPB-001-25

Super piggyBac™ transposase (sPBo) expression vector is a hyperactive piggyBac™ transposase that facilitates stable integration of small and large genes into target genomes.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 25µg size.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

sPBo-50
$1476.00
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Super piggyBac™ transposase expression vector, CMV promoter, 50µg size (sPBo-50)

Catalog #: SPB-001-50

Super piggyBac™ transposase (sPBo) expression vector is a hyperactive piggyBac™ transposase that facilitates stable integration of small and large genes into target genomes.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 50µg size.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

PBx-10
$369.00
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Excision-only piggyBac™ transposase expression vector, 10µg size (PBx-10)

Catalog #: SPB-002-10

Excision-only piggyBac™ transposase (PBx) expression vector is a transposase for excision of piggyBac™ selection cassettes and transgenes for Footprint-Free™ gene editing or phenotype reversions.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 10µg size.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

PBx-25
$830.00
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Excision-only piggyBac™ transposase expression vector, 25µg size (PBx-25)

Catalog #: SPB-002-25

Excision-only piggyBac™ transposase (PBx) expression vector is a transposase for excision of piggyBac™ selection cassettes and transgenes for Footprint-Free™ gene editing or phenotype reversions.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 25µg size.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

pbx-50
$1,476.00
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Excision-only piggyBac™ transposase expression vector, 50µg size (PBx-50)

Catalog #: SPB-002-50

Excision-only piggyBac™ transposase (PBx) expression vector is a transposase for excision of piggyBac™ selection cassettes and transgenes for Footprint-Free™ gene editing or phenotype reversions.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 50µg size.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

sPBo-m
$599.00
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Super piggyBac™ transposase mRNA (sPBo-m)

Catalog #: SPB-003

Super piggyBac™ transposase mRNA. The piggyBac™ transposase mRNA is recommended for cells that are not efficiently transfected with vector DNA.

  • 25µg size.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

PBx-m
$599.00
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Excision-only piggyBac™ transposase mRNA (PBx-m)

Catalog #: SPB-004

Excision-only piggyBac™ transposase mRNA. The Excision-only piggyBac™ transposase mRNA is recommended for cells that are not efficiently transfected with vector DNA.

  • 25µg size.
  • Estimated delivery is 3-7 business days.
  • Contact us for bulk orders (>250µg).

PBNeo
$499.00
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piggyBac™ transposon vector with Neomycin resistance that is driven by the SV40 Promoter and transcription is terminated by a SV40 PolyA signal (PBNeo)

Catalog #: SPB-005

Customizable piggyBac™ transposon vector contains an SV40 promoter-driven neomycin resistance gene. An insulator-flanked MCS allows for cloning in elements of interest, including user chosen genes driven by any selected promoter. The core insulators will protect cargo from genomic position effects and gene silencing.

  • This vector is renewable (Neomycin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
PB001
$499.00
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piggyBac™ transposon vector with Polr2A promoter-MCS-TKpA (PB001)

Catalog #: SPB-006

Customizable piggyBac™ transposon vector contains a Polr2A promoter for ubiquitous gene expression. This promoter contains an intron and has demonstrated excellent expression levels in vivo and in transgenic animal models.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
PB001G
$499.00
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piggyBac™ transposon with Polr2A promoter with MCS, GFP and TK polyA transcription termination (PB001G)

Catalog #: SPB-009

Similar to SPB-006, but SPB-009 provides the flexibility to clone any gene or cDNA with an in-frame GFP-fusion marker. This vector can also serve as a positive control for transposition.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
PB007
$499.00
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piggyBac™ transposon, no GFP. Has TK polyA transcription termination (PB007)

Catalog #: SPB-007

This customizable piggyBac™ transposon vector contains an insulator-flanked large multiple cloning site (MCS), allowing the flexibility to clone in your promoter and DNA cargo of choice. The core insulators protect cargo from genomic position effects and gene silencing.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
PB007G
$499.00
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piggyBac™ transposon with GFP fluorescent marker to track gene expression. Has TK polyA transcription termination (PB007G)

Catalog #: SPB-008

Similar to SPB-007, but SPB-008 contains a GFP gene preceded by cloning sites that allow cloning of your promoter and gene of interest in frame to monitor expression.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
PB002G
$499.00
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piggyBac™ transposon with CAG promoter, MCS, GFP, and TK polyA transcription termination (PB002G)

Catalog #: SPB-010

This customizable piggyBac™ transposon vector contains the strong CAG promoter driving GFP expression. This vector also features an MCS to clone an expression cassette of your choice. The GFP marker will be driven independently from your cargo.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
PB003G
$499.00
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piggyBac™ transposon with CMV promoter, MCS, GFP, and TK polyA transcription termination (PB003G)

Catalog #: SPB-011

Similar to SPB-010, but SPB-011 replaces the CAG promoter with a CMV promoter to express GFP

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
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$399.00
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piggyBac™ splinkerette PCR Kit (PB-SPLK-1)

Catalog #: KPB-001

Optimized protocol and verified primers required for identification of piggyBac™ transposon integration sites.