Bring the Footprint-Free™ gene editing system to your lab

Transposagen is the only commercial service provider offering comprehensive Footprint-Free™ Gene Editing Kits. Our kits select for gene editing events without leaving scarring sequences behind. This proven approach to editing means less screening, shorter timelines, and a higher probability of success with full confidence in assays and results.

How it Works

Graphic: How it works

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Comparison chart to ssODNS, Cre/LoxP, HR only

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Application: Genome Engineering

  Cre/lox or Flp/Frt with selectionLoxP/Cre ssODN piggyBac with selection
Targeting efficiency high low high
Footprint 34bp 1-3 bp None, if TTAA close enough
Timeline moderate Fast if automation and process in place Slower due to excsision step
Knock-in size 1bp to kilobases ~35 bp 1bp to kilobases

Transposagen’s Footprint-Free™ gene editing kits and services combine the site-specific nucleases, with our exclusive piggyBac™ transposon system––the only commercially available method capable of seamless excision of resistance or reporter genes.

Footprint-Free™ Gene Editing allows users to increase efficiencies by utilizing selection enrichment for gene edits, followed by “footprint-free” or “scarless” removal of the selection cassette, resulting in the cleanest genome edit.

Advantages of Footprint-Free™ Gene Editing

Why selection? Site-specific nucleases increase the efficiency of targeted knock-ins or gene edits significantly. In many cases, however, the efficiency may not be high enough for clone isolation. The ability to select for desired mutations and rare events is essential for efficient gene editing.

Selection vs. Single-Stranded DNA Oligonucleotide

One alternative to selection is the use of ssODNs which are typically 60-120 bases with the donor substitution (base-pair edits), flanked by homologous sequences on each side. Inefficiencies require a large number of clones to be screened resulting in project timeline delays and cost increases. Footprint-Free™ selection enrichment allows simpler, easier screening, for faster and more cost effective genome editing.

Footprint-Free™ Gene Editing vs. CRE/loxP Recombinase

Recombinase methods remove drug selection markers from the genome by catalyzing recombination between target sites (known as loxP sites). However, this process is not clean and leaves “footprints” or “scars” in the genome which have been shown to disturb splicing elements or alter/silence gene expression. In addition, recombinases recognize “cryptic” loxP sites and produce off-target mutations, compromising results. Footprint-Free™ gene editing does not have these adverse effects, providing precise and efficient genome editing for quality results.

Our in-house experts design and deliver, making gene editing in your lab that much easier.

Footprint-Free™ gene editing kits

Off-The-Shelf Reagents

MV-CMV-GFP-Puro SGK-001 Map
$699.00
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Empty Multivector containing GFP and Puromycin selection cassette driven by a CMV promoter (MV-CMV-GFP-Puro)

Catalog #: SGK-001

The Multivector is used for Footprint-Free™ gene editing, which allows for the selection of genome modifications and seamless removal of the selection cassette with the Excision-only piggyBac™ transposase. The piggyBac™ ITRs flank a CMV driven GFP and Puromycin resistant dual selection cassette. Cloning sites allow for custom cloning of homologous targeting arms.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
MV-PGK-GFP-Puro SGK-002 Map
$699.00
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Empty Multivector containing GFP and Puromycin selection cassette driven by a PGK promoter (MV-PGK-GFP-Puro)

Catalog #: SGK-002

The Multivector is used for Footprint-Free™ gene editing, which allows for the selection of genome modifications and seamless removal of the selection cassette with the Excision-only piggyBac™ transposase. The piggyBac™ ITRs flank a PGK driven GFP and Puromycin resistant dual selection cassette. Cloning sites allow for custom cloning of homologous targeting arms.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
MV-PGK-Neo SGK-003 Map
$699.00
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Empty Multivector containing a Neomycin selection cassette driven by a PGK promoter (MV-PGK-Neo)

Catalog #: SGK-003

The Multivector is used for Footprint-Free™ gene editing, which allows for the selection of genome modifications and seamless removal of the selection cassette with the Excision-only piggyBac™ transposase. The piggyBac™ ITRs flank a PGK driven Neomycin resistant dual selection cassette. Cloning sites allow for custom cloning of homologous targeting arms.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
MV-PGK-Neo-TK SGK-004 Map
$699.00
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Empty Multivector containing Neomycin and Thymidine Kinase selection cassette driven by a PGK promoter (MV-PGK-Neo-TK)

Catalog #: SGK-004

The Multivector is used for Footprint-Free™ gene editing, which allows for the selection of genome modifications and seamless removal of the selection cassette with the Excision-only piggyBac™ transposase. The piggyBac™ ITRs flank a PGK driven Neomycin resistance and Thymidine Kinase dual selection cassette. Cloning sites allow for custom cloning of homologous targeting arms.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
MV-PGK-Puro-TK SGK-005 Map
$699.00
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Empty Multivector containing Puromycin and Thymidine Kinase selection cassette driven by a PGK promoter (MV-PGK-Puro-TK)

Catalog #: SGK-005

The Multivector is used for Footprint-Free™ gene editing, which allows for the selection of genome modifications and seamless removal of the selection cassette with the Excision-only piggyBac™ transposase. The piggyBac™ ITRs flank a PGK driven Puromycin resistance and Thymidine Kinase dual selection cassette. Cloning sites allow for custom cloning of homologous targeting arms.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
MV-PGK-Hygro-TK SGK-006 Map
$699.00
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Empty Multivector containing Hygromycin and Thymidine Kinase selection cassette driven by a PGK promoter (MV-PGK-Hygro-TK)

Catalog #: SGK-006

The Multivector is used for Footprint-Free™ gene editing, which allows for the selection of genome modifications and seamless removal of the selection cassette with the Excision-only piggyBac™ transposase. The piggyBac™ ITRs flank a PGK driven Hygromycin resistance and Thymidine Kinase dual selection cassette. Cloning sites allow for custom cloning of homologous targeting arms.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
MV-EF1a-Puro-TK SGK-007 Map
$699.00
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Empty Multivector containing Puromycin and Thymidine Kinase selection cassette driven by an EF1α promoter (MV-EF1α-Puro-TK)

Catalog #: SGK-007

The Multivector is used for Footprint-Free™ gene editing, which allows for the selection of genome modifications and seamless removal of the selection cassette with the Excision-only piggyBac™ transposase. The piggyBac™ ITRs flank an EF1α driven Puromycin resistance and Thymidine Kinase dual selection cassette. Cloning sites allow for custom cloning of homologous targeting arms.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.