High efficiency, easy to use reagent options for gene editing

Site-specific CRISPR nucleases enable researchers to accurately cut any DNA sequence, allowing for the creation of gene knock-outs, knock-ins and single base pair edits. Transposagen offers a wide variety of CRISPR nucleases including the original CRISPR system, our NextGEN™ dimeric CRISPR and the all-in-one Cas9+gRNA expression system.

Wildtype CRISPR

The wildtype CRISPR technology uses a catalictly active Cas9 protein guided by a programmable guide RNA molecule to make targeted double strand breaks in the genome.

Off-The-Shelf Reagents

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$199.00
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Cas9 nuclease expression vector, CMV promoter (Cas9)

Catalog #: SWC-001

CMV expressed Cas9 nuclease vector.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 25µg size.
  • Estimated delivery is 3-7 business days.
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$399.00
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Cas9 nuclease mRNA (mCas9)

Catalog #: SWC-002

High purity, transfection ready, Cas9 mRNA in nuclease free water.

  • 25µg size.
  • Estimated delivery is 3-7 business days.

SingleShot All-in-One CRISPR

The All-in-One SingleShot CRISPR expresses the Cas9 and guide RNA off a single plasmid to simplify cell transfection protocols.

CRISPR xPB™ Off-the-Shelf Reagents

CRISPR-xPB-CMV-Puro-TK SCP-001 Map
$999.00
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piggyBac™ transposon expressing Cas9, CMV promoter. Contains Puromycin resistance and Thymidine Kinase selection cassette (CRISPR-xPB™-CMV-Puro-TK)

Catalog #: SCP-001

piggyBac™ transposon expressing Cas9 driven by CMV promoter. Contains Puromycin resistance gene and Thymidine Kinase (TK) selection cassette. This piggyBac™ transposon vector is used to non-specifically integrate and express the Cas9 protein. Puromycin resistance and TK cassette allows for selection of integration events, and, if desired, seamless removal of the entire Cas9/selection cassette with Excision-only piggyBac™ transposase.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
CRISPR-xPB-dCas9-Cag-Puro-TK SCP-002 Map
$999.00
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piggyBac™ transposon expressing catalytically inactive Cas9 (dCas9), CAG promoter. Contains Puromycin resistance and Thymidine Kinase selection cassette (CRISPR-xPB™-dCas9-CAG-Puro-TK)

Catalog #: SCP-002

piggyBac™ transposon expressing catalytically inactive Cas9 (dCas9) driven by CAG promoter. Contains Puromycin resistance gene and Thymidine Kinase (TK) selection cassette. This piggyBac™ transposon vector is used to non-specifically integrate and express the catalytically inactive Cas9 protein. Puromycin resistance and TK selection cassette allows for selection for integration, and, if desired, seamless removal of the entire dCas9/selection cassette with Excision-only piggyBac™ transposase.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.

NextGEN™ CRISPR

Earlier generations of site-specific nucleases, such as the wildtype CRISPR/Cas9 system can create off-target mutations. The NextGEN™ CRISPR technology is a dimeric RNA-guided type IIS nuclease which significantly diminishes these concerns. The NextGEN™ CRISPR system empowers researchers to engineer the genome with the highest fidelity without compromising the budget.

Off-The-Shelf Reagents

NextGEN™ dCas9-CMV
$199.00
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NextGEN™ dCas9-Type IIS nuclease vector, CMV promoter (NextGEN™ dCas9-CMV)

Catalog #: SNC-001

CMV expressed catalytically inactive Cas9 with an N-terminal bound, dimerization-dependent, Type IIS endonuclease. NextGEN™ dCas9-Type IIS vectors must be used with NextGEN™ CRISPR gRNAs Cat# CNC-001.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 25µg size.
  • Estimated delivery is 3-7 business days.
NextGEN™ dCas9-EF1alpha
$199.00
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NextGEN™ dCas9-Type IIS nuclease vector, EF1alpha promoter (NextGEN™ dCas9-EF1alpha)

Catalog #: SNC-002

EF1alpha expressed catalytically inactive Cas9 with an N-terminal bound, dimerization-dependent, Type IIS endonuclease. NextGEN™ dCas9-Type IIS vectors must be used with NextGEN™ CRISPR gRNAs Cat# CNC-001.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 25µg size.
  • Estimated delivery is 3-7 business days.
NextGEN dCas9-CAG
$199.00
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NextGEN™ dCas9-Type IIS nuclease vector, CAG promoter (NextGEN™ dCas9-CAG)

Catalog #: SNC-003

CAG expressed catalytically inactive Cas9 with an N-terminal bound, dimerization-dependent, Type IIS endonuclease. NextGEN™ dCas9-Type IIS vectors must be used with NextGEN™ CRISPR gRNAs Cat# CNC-001.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 25µg size.
  • Estimated delivery is 3-7 business days.