High efficiency, easy to use reagent options for gene editing

Site-specific CRISPR nucleases enable researchers to accurately cut any DNA sequence, allowing for the creation of gene knock-outs, knock-ins and single base pair edits. The SingleShot all-in-one Cas9+gRNA expression system combined with seamless piggyBac® removal enables simplified Footprint-Free® gene editing as described in Nature Protocols 2017.

SingleShot All-in-One CRISPR

The All-in-One SingleShot CRISPR expresses the Cas9 and guide RNA off a single plasmid to simplify cell transfection protocols.

CRISPR-xPB-CMV-Puro-TK SCP-001 Map
$999.00
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piggyBac transposon expressing Cas9, CMV promoter. Contains Puromycin resistance and Thymidine Kinase selection cassette (CRISPR-xPB-CMV-Puro-TK)

Catalog #: SCP-001

piggyBac transposon expressing Cas9 driven by CMV promoter. Contains Puromycin resistance gene and Thymidine Kinase (TK) selection cassette. This piggyBac transposon vector is used to non-specifically integrate and express the Cas9 protein. Puromycin resistance and TK cassette allows for selection of integration events, and, if desired, seamless removal of the entire Cas9/selection cassette with Excision-only piggyBac transposase.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
CRISPR-xPB-dCas9-Cag-Puro-TK SCP-002 Map
$999.00
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piggyBac transposon expressing catalytically inactive Cas9 (dCas9), CAG promoter. Contains Puromycin resistance and Thymidine Kinase selection cassette (CRISPR-xPB-dCas9-CAG-Puro-TK)

Catalog #: SCP-002

piggyBac transposon expressing catalytically inactive Cas9 (dCas9) driven by CAG promoter. Contains Puromycin resistance gene and Thymidine Kinase (TK) selection cassette. This piggyBac transposon vector is used to non-specifically integrate and express the catalytically inactive Cas9 protein. Puromycin resistance and TK selection cassette allows for selection for integration, and, if desired, seamless removal of the entire dCas9/selection cassette with Excision-only piggyBac transposase.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.