Predictable transgene expression in the AAVS1 Safe Harbor Site

Verified reagents to insert genes at the well-characterized AAVS1 Safe Harbor Site, with the option to later remove components using Cre or excision-only piggyBac™.

Advantages of the AAVS1 Safe Harbor Kit

  1. Characterized location for transgene insertion without disrupting cellular functions.
  2. The AAVS1 site protects transgenes from silencing.
  3. Some transgenic constructs targeted to the AAVS1 site maintain expression in cells differentiated from human induced pluripotent stem (iPS) cells.

Safe Harbor AAVS1 Reagents & Kits

Off-The-Shelf Reagents

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$999.00
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AAVS1 Safe-harbor kit: GFP-Puro with CRISPR (AAVS-G-CRISPR)

Catalog #: KSH-001

AAVS1 donor vector and AAVS1-specific CRISPR/Cas9 nuclease. The AAVS1 safe-harbor donor vector contains arms of homology to AAVS1, multiple cloning sites, and a GFP-Puromycin resistance gene selection cassette. The selection cassette is flanked by loxP sites and can be removed with CRE recombinase (not included) following targeted integration. The entire selection cassette and MCS region is flanked by piggyBac™ ITRs and can be seamlessly removed with Excision-only piggyBac™ transposase (not included) for phenotype reversion.

  • Kit includes 10µg of the AAVS1-specific donor vector, 10ug of an AAVS1-specific all-in-one CRISPR/Cas9 expression vector.
  • All vectors are renewable (Ampicillin resistance).
  • Estimated delivery is 3-7 business days.
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$999.00
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AAVS1 Safe-harbor kit: GFP-Puro with XTN™ TALEN (AAVS-G-TALEN)

Catalog #: KSH-002

AAVS1 donor vector and AAVS1-specific XTN™ TALEN nucleases. The AAVS1 safe-harbor donor vector contains arms of homology to AAVS1, multiple cloning sites, and a GFP-Puromycin resistance gene selection cassette. The selection cassette is flanked by loxP sites and can be removed with CRE recombinase (not included) following targeted integration. The entire selection cassette and MCS region is flanked by piggyBac™ ITRs and can be seamlessly removed with Excision-only piggyBac™ transposase (not included) for phenotype reversion.

  • Kit includes 10µg of the AVVS1-specific donor vector and 10ug of the AVVs1-specifc XTN™ TALEN expression vector.
  • All vectors are renewable (Ampicillin resistance).
  • Estimated delivery is 3-7 business days.
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$999.00
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AAVS1 Safe-harbor kit: Puro-TK with CRISPR (AAVS-P-CRISPR)

Catalog #: KSH-003

AAVS1 donor vector and AAVS1-specific CRISPR/Cas9 nuclease. The AAVS1 safe-harbor donor vector contains arms of homology to AAVS1, multiple cloning sites, and a Puromycin resistance gene and Thymidine Kinase (TK) selection cassette. The selection cassette is flanked by loxP sites and can be removed with CRE recombinase (not included) following targeted integration. The entire selection cassette and MCS region is flanked by piggyBac™ ITRs and can be seamlessly removed with Excision-only piggyBac™ transposase (not included) for phenotype reversion. The TK negative selection cassette allows for efficient removal of clones that did not successful excise their cargos.

  • Kit includes 10µg of the AAVS1-specific donor vector, 10ug of an AAVS1-specific all-in-one CRISPR/Cas9 expression vector.
  • All vectors are renewable (Ampicillin resistance).
  • Estimated delivery is 3-7 business days.
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$999.00
Request a quote

AAVS1 Safe-harbor kit: Puro-TK with XTN™ TALEN (AAVS-P-TALEN)

Catalog #: KSH-004

AAVS1 donor vector and AAVS1-specific XTN™ TALEN nucleases. The AAVS1 safe-harbor donor vector contains arms of homology to AAVS1, multiple cloning sites, and a Puromycin resistance gene and Thymidine Kinase selection cassette. The selection cassette is flanked by loxP sites and can be removed with CRE recombinase (not included) following targeted integration. The entire selection cassette and MCS region is flanked by piggyBac™ ITRs and can be seamlessly removed with Excision-only piggyBac™ transposase (not included) for phenotype reversion. The TK negative selection cassette allows for efficient removal of clones that did not successful excise their cargos.

  • Kit includes 10µg of the donor vector and 10ug of the XTN™ TALEN expression vector.
  • All vectors are renewable (Ampicillin resistance).
  • Estimated delivery is 3-7 business days.
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$999.00
Request a quote

AAVS1 Safe-harbor kit: MCS with CRISPR (AAVS1-CRISPR)

Catalog #: KSH-005

AAVS1 donor vector and AAVS1-specific CRISPR/Cas9 nuclease. The AAVS1 safe-harbor donor vector contains arms of homology to AAVS1, multiple cloning sites, and an empty selection cassette. The selection cassette is flanked by loxP sites and can be removed with CRE recombinase (not included) following targeted integration. The entire selection cassette and MCS region is flanked by piggyBac™ ITRs and can be seamlessly removed with Excision-only piggyBac™ transposase (not included) for phenotype reversion.

  • Kit includes 10µg of the AAVS1-specific donor vector, 10ug of an AAVS1-specific all-in-one CRISPR/Cas9 expression vector.
  • All vectors are renewable (Ampicillin resistance).
  • Estimated delivery is 3-7 business days.
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$999.00
Request a quote

AAVS1 Safe-harbor kit: MCS with XTN™ TALEN (AAVS1-TALEN)

Catalog #: KSH-006

AAVS1 donor vector and AAVS1-specific XTN™ TALEN nucleases. The AAVS1 safe-harbor donor vector contains arms of homology to AAVS1, multiple cloning sites, and an empty selection cassette. The selection cassette is flanked by loxP sites and can be removed with CRE recombinase (not included) following targeted integration. The entire selection cassette and MCS region is flanked by piggyBac™ ITRs and can be seamlessly removed with Excision-only piggyBac™ transposase (not included) for phenotype reversion.

  • Kit includes 10µg of the donor vector and 10ug of the XTN™ TALEN expression vector.
  • All vectors are renewable (Ampicillin resistance).
  • Estimated delivery is 3-7 business days.
AAVS1-CRISPR KSH-007 Map
$299.00
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CRISPR targeting AAVS1 site (AAVS1-CRISPR)

Catalog #: KSH-007

All-in-one vector that expresses both Cas9 and custom guide RNA (gRNA) targeting the AAVS1 site The Cas9 is expressed by a CMV promoter. The gRNA is driven separately by the U6 promoter.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
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$299.00
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TALEN targeting AAVS1 site (AAVS1-TALEN)

Catalog #: KSH-008

XTN™ TALEN expression vectors targeting the AAVS1 site.

  • This vector is renewable (Ampicillin resistance).
  • 10µg size.
  • Estimated delivery is 3-7 business days.
pAAVS-G KSH-009 Map
$899.00
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AAVS1 Safe-harbor donor vector: GFP-Puro (pAAVS-G)

Catalog #: KSH-009

AAVS1 donor. The AAVS1 safe-harbor donor vector contains arms of homology to AAVS1, multiple cloning sites, and a GFP-Puromycin resistance gene selection cassette. The selection cassette is flanked by loxP sites and can be removed with CRE recombinase (not included) following targeted integration. The entire selection cassette and MCS region is flanked by piggyBac™ ITRs and can be seamlessly removed with Excision-only piggyBac™ transposase (not included) for phenotype reversion.

  • Kit includes 10µg of the donor vector.
  • This vector is renewable (Ampicillin resistance).
  • Estimated delivery is 3-7 business days.
pAAVS-P KSH-010 Map
$899.00
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AAVS1 Safe-harbor donor vector: Puro-TK (pAAVS-P)

Catalog #: KSH-010

AAVS1 donor vector. The AAVS1 safe-harbor donor vector contains arms of homology to AAVS1, multiple cloning sites, and a Puromycin resistance gene and Thymidine Kinase selection cassette. The selection cassette is flanked by loxP sites and can be removed with CRE recombinase (not included) following targeted integration. The entire selection cassette and MCS region is flanked by piggyBac™ ITRs and can be seamlessly removed with Excision-only piggyBac™ transposase (not included) for phenotype reversion.

  • Kit includes 10µg of the donor vector.
  • This vectors is renewable (Ampicillin resistance).
  • Estimated delivery is 3-7 business days.
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$899.00
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AAVS1 Safe-harbor donor vector: MCS (pAAVS1)

Catalog #: KSH-011

Super piggyBac™ transposase (sPBo) expression vector is a hyperactive piggyBac™ transposase that facilitates stable integration of small and large genes into target genomes.

  • This vector is non-renewable, and cannot be propagated in bacteria.
  • 10µg size.
  • Estimated delivery is 3-7 business days.