Using Transposagen's piggyBac™ transposons, small to very large (200kb+) cargo sizes can be delivered to cells quickly with long-term stability, getting to results quicker.
When stable cell lines are needed, piggyBac™ can insert small to very large sequences and maintain stable expression for extended durations, surpassing many other options available today.
Table: Application Transgenesis
|piggyBac™||Retroviral||Transient Transfection||Stable Cell Generation||FLiP-In|
|Cargo Size||>250 kb||~4kb||~25kb||~25kb||250kb only one copy|
|Ease of cloning||Easy||easy||easy||easy||easy|
|Time to result||fast||Two step; must make virus once cloning done||Fast but fleeting||Same as pBo||Two step if making own flp cells|
|Stability of expression||good||Silencing common||none||variable||Good if frt site is well placed|
|Applicable to any cell?||no||yes||no||no||no|
Fast, Efficient & Versatile
piggyBac™ protein expression exceeds conventional methods even without selection. The piggyBac™ transposon increases protein production through preferential insertion into highly-transcribed regions of the genome while inverted terminal repeats (ITR) provide insulation from gene silencing, leading to higher expression over time.
Chart: piggyBac™ vs. Plasmid comparison
Engineered cell line, non-specific transgenic gene knock-inCatalog #: Tgcell
For a detailed description of this service, and accurate pricing, please contact us for a customized quote. Timelines and pricing will depend on complexity of gene edit and cell type. Estimated delivery of single cell clones is approximately 3-5 months.