- Products & Services
- Cell Engineering Services
- Stable Cell Line
Using Transposagen's piggyBac™ transposons, small to very large (200kb+) cargo sizes can be delivered to cells quickly with long-term stability, getting to results quicker.
When stable cell lines are needed, piggyBac™ can insert small to very large sequences and maintain stable expression for extended durations, surpassing many other options available today.
Table: Application Transgenesis
piggyBac™ | Retroviral | Transient Transfection | Stable Cell Generation | FLiP-In | |
---|---|---|---|---|---|
Cargo Size | >250 kb | ~4kb | ~25kb | ~25kb | 250kb only one copy |
Ease of cloning | Easy | easy | easy | easy | easy |
Parallel processing | |||||
Time to result | fast | Two step; must make virus once cloning done | Fast but fleeting | Same as pBo | Two step if making own flp cells |
Stability of expression | good | Silencing common | none | variable | Good if frt site is well placed |
Safety concerns | none | Important | none | none | none |
Applicable to any cell? | no | yes | no | no | no |

Fast, Efficient & Versatile
piggyBac™ protein expression exceeds conventional methods even without selection. The piggyBac™ transposon increases protein production through preferential insertion into highly-transcribed regions of the genome while inverted terminal repeats (ITR) provide insulation from gene silencing, leading to higher expression over time.
Chart: piggyBac™ vs. Plasmid comparison

Engineered cell line, non-specific transgenic gene knock-in
Catalog #: TgcellFor a detailed description of this service, and accurate pricing, please contact us for a customized quote. Timelines and pricing will depend on complexity of gene edit and cell type. Estimated delivery of single cell clones is approximately 3-5 months.