Transposagen utilizes three site-specific nuclease technologies––XTN™ TALEN, CRISPR, and NextGEN™ CRISPR––to maximize cell knock-out success.
Using site-specific nucleases, Transposagen can knock-out genes with high efficiency. Using the newer dimeric CRISPR build (NextGEN), we produce targeted knock-outs without the off-target problems common among earlier technologies.
Comparison chart of nuclease options
|Wt CRISPR||Dimeric NextGEN™ CRISPR||TALEN|
|Availability of anchor sequence||Frequent||Less frequent||Plentiful|
|Off-Target effects||Moderate||Not detectable||Low|
|Build time||1-2 weeks||1-2 weeks||2-4 weeks|
Engineered cell line, specific gene knock-outCatalog #: KOcell
For a detailed description of this service, and accurate pricing, please contact us for a customized quote. Timelines and pricing will depend on complexity of gene edit and cell type. Estimated delivery of single cell clones is approximately 4-6 months.