A Basic Guide to Reducing Risk in Gene Editing Projects

Even equipped with the most advanced tools and techniques, gene editing projects still carry inherent risk. While no lab can guarantee a successful outcome at the onset of a project, sound planning and a full understanding of the challenges you can expect to face can help set more realistic expectations and prevent wasteful costs when projects don’t pan out.

Taking this one step further, we’ve developed a milestone-based approach to project development to better reflect the hard-to-predict nature of genomic manipulation.

Rather than swallow the bitter pill of a massive upfront cost without any insight into the chances of success, we recognize the risks at each phase of development and address them through honest communication and a sensible pricing model.

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Rats vs. Mice in Genetic Rodent Model Engineering

Mice and rats have been helping scientists investigate human disease for well over a century, playing a crucial role in the discovery of treatments and therapeutics. Since their widespread introduction to the laboratory decades ago, rodents still make up 
95% of the animal models used in biomedical research today.

And while mice have traditionally been the primary model of choice for most research in the lab, recent advances have expanded the testing capabilities of the rat, whose larger size and varied characteristics allow for greater procedural manipulation better suited for many newly emerging therapeutic areas.

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4 Advantages of Footprint-Free Gene Editing in iPSCs & Stem Cells

Over just the past few years, new gene editing technologies have opened the door to more rapid innovations in genetic research and therapeutics. But even advanced site-specific nucleases like CRISPR/Cas9 and TALENs can still produce low frequency results, especially in difficult cell lines like stem cells and induced pluripotent stem cells (iPSCs).

As a result, scientists rely on selection methods to achieve their desired edits. Unfortunately, these methods typically leave genetic remnants behind––hindering progress for the many beneficial applications gene editing offers. As the demand for more precise and efficient selection methods has grown, a new system provides exactly the kind of clean editing capabilities scientists need.

Footprint-Free™ gene editing combines the excision-only piggyBac™ transposase with the latest site-specific nuclease technologies like CRISPR and XTN™ TALEN to select for very rare events and surgically alter single nucleotides without creating unwanted mutations.

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piggyBac Transposons: An Easier Solution to Gene Editing & Stable Cell Line Generation

Site-specific nucleases like CRISPR/Cas9, TALENs, and ZFNs have made gene editing more efficient and cost-effective than ever before. However, challenges still stand in the way of achieving seamless gene editing and isolating specifically-edited cells.

Similar obstacles also lay ahead when generating stable cell lines. The cumbersome process of transfecting, selecting, and isolating clones relies heavily on random integration efficiencies that can be low in the cell line of interest, making for an all around unreliable system. 

Fortunately, scientists and researchers are overcoming these hurdles with the piggyBac System––a plasmid that, when combined with a source of piggyBac transposase, allows for fast and efficient transference of a DNA sequence from the transposon vector to ‘TTAA’ sequences located throughout the genome.

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